Plasmid
pUC19 ori
Part:BBa_I50020:Design
Designed by Reshma Shetty Group: Knight Lab, MIT (2006-01-09)
From partsregistry.org
Minimal pUC19-derived high copy replication origin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 19
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
Design Notes
This part was designed to be a near minimal pUC19-derived high copy replication origin for use in the designed BioBrick base vector BBa_I51000. Several mutations were introduced to eliminate useful restriction sites.
Source
pSB1A3 served as the basis of the design of BBa_I50022.
References
- Vieira J and Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 1982 Oct; 19(3) 259-68. pmid:6295879.
- Norrander J, Kempe T, and Messing J. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene 1983 Dec; 26(1) 101-6. pmid:6323249.
- Yanisch-Perron C, Vieira J, and Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 1985; 33(1) 103-19. pmid:2985470.
- Cabello F, Timmis K, and Cohen SN. Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons. Nature 1976 Jan 29; 259(5541) 285-90. pmid:765836.
- Ioannou PA, Amemiya CT, Garnes J, Kroisel PM, Shizuya H, Chen C, Batzer MA, and de Jong PJ. A new bacteriophage P1-derived vector for the propagation of large human DNA fragments. Nat Genet 1994 Jan; 6(1) 84-9. doi:10.1038/ng0194-84 pmid:8136839.
