DNA
F plasmid
Part:BBa_I50000:Design
Designed by Reshma Shetty Group: Knight Lab, MIT (2005-11-16)
From partsregistry.org
F plasmid backbone with BioBrick sites removed.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 133
Illegal BglII site found at 2200 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 690
Illegal NgoMIV site found at 4606
Illegal AgeI site found at 814
Illegal AgeI site found at 2044
Illegal AgeI site found at 2937
Design Notes
Source
References
- J. Wild, Z. Hradecna, and W. Szybalski. Conditionally amplifiable BACs: switching from single-copy to high-copy vectors and genomic clones. Genome Res, 12(1088-9051):1434,44, 2002. (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12213781&query_hl=4)
- pSMART VC vector from Lucigen http://www.lucigen.com/products/Cloning_Systems/CopyRight.html. GenBank accession number AY643800
