Part:BBa_I50000

From partsregistry.org

F plasmid backbone with BioBrick sites removed.

This is a (near) single copy origin derived from the F plasmid. The naturally occurring BioBricks sites were removed with an intent to preserve the amino acid and codon frequency where possible. Similar to the pSMART VC sequence from Lucigen http://www.lucigen.com/products/Cloning_Systems/CopyRight.html. It is intended to be combined with other vector components (antibiotic resistance marker, multiple cloning site) in order to form a BioBricks vector. Relevant information can be found at http://openwetware.org/wiki/BioBrick_Parts_for_Plasmid_Engineering.

Usage and Biology

-- Deleted --

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 133
Illegal BglII site found at 2200
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 690
Illegal NgoMIV site found at 4606
Illegal AgeI site found at 814
Illegal AgeI site found at 2044
Illegal AgeI site found at 2937

Retrieved from "http://partsregistry.org/Part:BBa_I50000"

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