1 Registry Star
Designed by: Barry Canton [email@example.com] and Anna Labno [firstname.lastname@example.org] Group: MIT (2004-08-09)
A transcription factor, LuxR (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by a TetR (BBa_C0040) regulated operator (BBa_R0040). Device input is 3OC6HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as aTc can be used to produce a logical AND function. Get the pdf datasheet here.
1Measured by Ania Labno and Barry Canton 2006-2007
Contribution: Arizona_State 2016
Authors: Ernesto Luna, Brady Dennison, Cassandra Barrett, Jimmy Xu, Jiaqi Wu, Dr. Karmella Haynes
The 2016 Arizona_State team ran a series of induction tests on 10 AHL Senders (naturally-produced by an BL21 E.coli chassis) and 5 synthetic AHL variants using Bba_F2620 as the output device. This included a series of visual plate induction tests as well as an 8-hour GFP absorbance read. This contribution aims to expand the characterization of this part by identifying crosstalk partners as well as potential orthogonal systems.
The table below summarized the findings of our induction test. Note: these results are not conclusive and are simply what the collected data indicated from our 8-hour induction test.
Two runs at two different concentrations (100nM and 1uM) using synthetic AHLs yielded some interesting differences, with synthetic p-Coumaroyl showing no induction while synthetic C12-HSL did induce. More in-depth figures and data can be found under the Experience tab of this part.