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From partsregistry.org
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_E0840
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is commonly used to quantify the function of transcriptional control devices.
User Reviews
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Content-type: text/html Software error:Global symbol "@ids" requires explicit package name at /web-site/partsregistry.org/cgi/lib/Scar.pm line 54. Compilation failed in require at /web-site/partsregistry.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/lib/Part.pm line 16. Compilation failed in require at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8. For help, please send mail to this site's webmaster, giving this error message and the time and date of the error. Smelissali |
Very visible even without UV excitation on plate and in solution after 24 hours incubation. Not as visible as Part:BBa_I13521 (mRFP), however. |
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Content-type: text/html Software error:Global symbol "@ids" requires explicit package name at /web-site/partsregistry.org/cgi/lib/Scar.pm line 54. Compilation failed in require at /web-site/partsregistry.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/lib/Part.pm line 16. Compilation failed in require at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8. For help, please send mail to this site's webmaster, giving this error message and the time and date of the error. |
Content-type: text/html Software error:Global symbol "@ids" requires explicit package name at /web-site/partsregistry.org/cgi/lib/Scar.pm line 54. Compilation failed in require at /web-site/partsregistry.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/lib/Part.pm line 16. Compilation failed in require at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8. For help, please send mail to this site's webmaster, giving this error message and the time and date of the error. was successfully used to quantify the behavior of Content-type: text/html Software error:Global symbol "@ids" requires explicit package name at /web-site/partsregistry.org/cgi/lib/Scar.pm line 54. Compilation failed in require at /web-site/partsregistry.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/lib/Part.pm line 16. Compilation failed in require at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8. For help, please send mail to this site's webmaster, giving this error message and the time and date of the error. , Content-type: text/html Software error:Global symbol "@ids" requires explicit package name at /web-site/partsregistry.org/cgi/lib/Scar.pm line 54. Compilation failed in require at /web-site/partsregistry.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/lib/Part.pm line 16. Compilation failed in require at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8. For help, please send mail to this site's webmaster, giving this error message and the time and date of the error. and Content-type: text/html Software error:Global symbol "@ids" requires explicit package name at /web-site/partsregistry.org/cgi/lib/Scar.pm line 54. Compilation failed in require at /web-site/partsregistry.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/lib/Part.pm line 16. Compilation failed in require at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8. For help, please send mail to this site's webmaster, giving this error message and the time and date of the error. . |
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Content-type: text/html Software error:Global symbol "@ids" requires explicit package name at /web-site/partsregistry.org/cgi/lib/Scar.pm line 54. Compilation failed in require at /web-site/partsregistry.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/lib/Part.pm line 16. Compilation failed in require at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8. For help, please send mail to this site's webmaster, giving this error message and the time and date of the error. Aberdeen_Scotland 2009 |
Our miniprep, double and single digests worked as expected. We used this biobrick for building two of our parts (Content-type: text/html Software error:Global symbol "@ids" requires explicit package name at /web-site/partsregistry.org/cgi/lib/Scar.pm line 54. Compilation failed in require at /web-site/partsregistry.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/lib/Part.pm line 16. Compilation failed in require at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8. For help, please send mail to this site's webmaster, giving this error message and the time and date of the error. and 'Content-type: text/html Software error:Global symbol "@ids" requires explicit package name at /web-site/partsregistry.org/cgi/lib/Scar.pm line 54. Compilation failed in require at /web-site/partsregistry.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/lib/Part.pm line 16. Compilation failed in require at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /web-site/partsregistry.org/cgi/partsdb/putout.cgi line 8. For help, please send mail to this site's webmaster, giving this error message and the time and date of the error. ). We experienced very satisfactory cloning with high efficiency of transformants. The sequencing was correct. We discovered that all the bacteria containing this biobrick were releasing fluorescence even though there was no promoter upstream. The level of fluorescence observed was dependent on the plasmid copy number. We noticed that colonies displayed a visible, yellow-green colour, therefore selection was very easy. |
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Characterization
Transcriptional control of GFP generator
All three transcriptional control devices were quantified using Content-type: text/html
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We successfully designed, constructed and tested transcriptional control devices for constitutive, stationary phase dependent and exponential phase dependent protein production (A-C). To test and verify function of our three transcriptional control devices, we assembled each control device with the GFP protein generator Content-type: text/html
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and monitored the fluorescence of E. coli cultures with each device over time. For each device, we plot the change in fluorescence per unit time (normalized GFP synthesis rate) versus the cell density (OD600nm) (D). The constitutive transcriptional control device produced a high GFP synthesis rate irrespective of cell density. The stationary phase transcriptional control device produced a low initial GFP synthesis rate which increased with culture cell density. The exponential phase transcriptional control device produced an initially high GFP synthesis rate which dropped off as cell density increased. Data shown are averages of triplicate measurements of cultures grown from three individual colonies of each device. Error bars are the standard deviation of the three individual cultures.
