1 Registry Star
Part:BBa_E0040:Design
From partsregistry.org
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
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Design Notes
GFPmut3* has a couple of mutations from wtGFP - S2R and S65G and S72A. Emerald and EGFP have S65T and multiple other mutations. The S2R mutation should have no effect.
Source
This part was derived from the reporter device used by Elowitz's repressilator [1]. Elowitz obtained the GFP tagged sequence (gfpmut3*) published by Andersen and coworkers [2] to build the reporter device. Andersen constructed those GFP variants based on a gfpmut3b containing plasmid that was a gift from R. H. Valdivia [3]. The PCR method used in construction by Andersen changed aa residue 2 from serine to arginine with no detectable phenotypic effect in their experiments.
References
- Elowitz MB and Leibler S. A synthetic oscillatory network of transcriptional regulators. Nature 2000 Jan 20; 403(6767) 335-8. doi:10.1038/35002125 pmid:10659856.
- Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, and Molin S. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl Environ Microbiol 1998 Jun; 64(6) 2240-6. pmid:9603842.
- Cormack BP, Valdivia RH, and Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 1996; 173(1 Spec No) 33-8. pmid:8707053.
