cI repressor from phage 434 (+LVA)
The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, Part:BBa_R0052. The sequence contains a LVA tag for faster degradation and has no RBS.
Usage and Biology
434 cI is a member of the lamboid cI protein family.
The coding sequence has been modified from wild type cI to contain an LVA tag and two TAA stop codons; its EcoRI recognition site has been mutated out by changing base 36 from T to C to be BioBrick-compatible.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 688
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC