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RBS

Part:BBa_B0030:Design

Designed by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.   Group: NCBS, Prather Lab

From partsregistry.org


RBS.1 (strong) -- modified from R. Weiss

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]


Design Notes

Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis).

No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.

Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>


Source

References