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RBS

Part:BBa_B0030

Designed by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.   Group: NCBS, Prather Lab   (2003-01-31)

From partsregistry.org

RBS.1 (strong) -- modified from R. Weiss

Strong RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034.


Usage and Biology

Note: not compatible with R0053 promoter due to likely transcript secondary structure This combination yielded very low gfp expression (see BBa_I7108). [jcb 8/3/05].

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]

Team Warsaw 2010's measurement

RBS strength (relative to B0034): 91,84%

Functional Parameters

biology-NA-
efficiency0.6
(Relative to BBa_B0034)
Retrieved from "http://partsregistry.org/Part:BBa_B0030"
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Categories
//rbs/prokaryote/constitutive/community
//ribosome/prokaryote/ecoli
//chassis/prokaryote/ecoli
//direction/forward
//regulation/constitutive
Reviews
1 Registry Star
Experience: Works
5
(77)
4
(21)
3
(44)
2
(13)
1
(5)
Twins
BBa_K783047 Planning
Parameters
biology
efficiency0.6