DNA Available

1 Registry Star

Terminator

Part:BBa_B0015

Designed by Reshma Shetty Group: Registry (2003-07-17)

From partsregistry.org

biology	rrnBT1-T7TE
chassis	-NA-
direction	Forward
forward_efficiency	0.984[CC] 0.97[JK]
reversed_version	2146
reverse_efficiency	0.295[CC] 0.62[JK]

double terminator (B0010-B0012)

Double terminator consisting of BBa_B0010 and BBa_B0012.
This is the most commonly used terminator. It seems to be reliable.
Note, however, that Part:BBa_B0014 is a better part for forward and reverse termination.

B0010	B0012

Secondary Structure

The mfold results are annotated with the location of the subparts BBa_B0010 and BBa_B0012 and the BioBrick assembly scar.

Using primers VR/VF2 to PCR B0010 will result in excess bands.

See a full description of the problem here.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]

Measurement

How these parts were measured

Retrieved from "http://partsregistry.org/Part:BBa_B0015"

[edit]

Categories

//direction/forward
//function/coliroid
//terminator/double

Reviews

1 Registry Star

Experience: Works

5

(77)

4

(21)

3

(44)

2

(13)

1

(5)

Twins

BBa_I714006 Planning
BBa_S03932 Planning
BBa_S04211 Planning
BBa_K185014 Sent
BBa_K185015 Sent
BBa_K185020 Planning
BBa_K185021 Planning
BBa_K185022 Planning
BBa_K185023 Planning
BBa_K185024 Planning
BBa_K185025 Planning
BBa_K185026 Planning
BBa_K185027 Planning
BBa_S04353 Planning

Parameters

biology	rrnBT1-T7TE
chassis
direction	Forward
forward_efficiency	0.984[CC] 0.97[JK]
reversed_version	2146
reverse_efficiency	0.295[CC] 0.62[JK]