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Terminator

Part:BBa_B0015

Designed by Reshma Shetty   Group: Registry   (2003-07-17)

From partsregistry.org

Terminator.png

biologyrrnBT1-T7TE
chassisE.coli
directionForward
forward_efficiency0.984[CC]
0.97[JK]
reversed_version2146
reverse_efficiency0.295[CC]
0.62[JK]

double terminator (B0010-B0012)

  • Double terminator consisting of BBa_B0010 and BBa_B0012.
  • This is the most commonly used terminator. It seems to be reliable.
    Note, however, that Part:BBa_B0014 is a better part for forward and reverse termination.


B0010

B0012

Secondary Structure

The mfold results are annotated with the location of the subparts BBa_B0010 and BBa_B0012 and the BioBrick assembly scar.

Mfold-B0015-1.png


Using primers VR/VF2 to PCR B0010 will result in excess bands.

See a full description of the problem here.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]

Measurement

[edit]
Categories
//direction/forward
//terminator/double
Reviews
1 Registry Star
Experience: Works
5
(77)
4
(21)
3
(44)
2
(13)
1
(5)
Twins
BBa_S03932 Deleted
BBa_S04211 Deleted
BBa_S04353 Deleted
BBa_K783020 Planning
Parameters
biologyrrnBT1-T7TE
chassisE.coli
directionForward
forward_efficiency0.984[CC]
0.97[JK]
reversed_version2146
reverse_efficiency0.295[CC]
0.62[JK]