From partsregistry.org
This page provides an overview of the wet-lab techniques you will need in order to manipulate the BioBrick DNA provided by the Registry.
Before You Start
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Things to consider before you actually begin wet-lab work...
Lab Hardware
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Lab Wetware
- Chemicals - under construction
- Agar/color coding
- Non-living substances for labwork - under construction
- Enzymes - under construction
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Resources
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| Our sister site Openwetware is a consortium of synthetic biology academic laboratories from around the country. This page will help point you in the right direction of particularly Assembly-relevant Protocols and Materials
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Other Online Resources
- Genetic engineering - Recommended especially for wet-lab beginners! Introduction to genetic engineering and cloning techniques.
- Molecular toolbox - extensive resource maintained at the Department of Molecular Genetics and Biochemistry, University of Pittsburgh
- Labwork's - guide to basic techniques in the lab
- Highveld's - directory for molecular biology, genomics, microbiology and PCR
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Creating a BioBrick
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You may be happy with the design of your new biobrick and have added it to your sandbox, but how do you go about physically making the part? Well, there are 2 approaches, each with their merits and pitfalls:
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PCR
Synthesis
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Using Your BioBricks at the Bench
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The Registry does not provide any protocols of its own--instead it refers to external sites (see Resources). However the following links should aid you greatly:
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Transformations
How to Perform a Construction
DNA extraction and purification
Ligation
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