Help:Adding Parts/Synthesis


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Synthesis and Adding a Part

We encourage teams to take advantage of DNA synthesis in their projects, for the past few years, IDT has put together a great offer for free synthesis for iGEM teams.

You may have used synthesis in your project to construct your device, either by synthesizing it entirely, or synthesizing large fragments that you've assembled together. This page will detail things to keep in mind as you add and document your synthesized part on the Registry.


BioBrick compatibility and pSB1C3

As you design your construct, make sure it is BioBrick compatible as that is a requirement for submission. You must also submit your construct in pSB1C3, so plan accordingly to ensure your construct will have/can maintain the BioBrick prefix and suffix.


Make sure to add your basic parts

If you synthesized your composite part, you may not have physical samples of the basic parts involved in your synthesized construct. You are not required to submit samples for the basic parts in your design, but you must still add and document these basic parts on the Registry because they will be needed to represent your composite part. Do not add a composite part as a sequence only!

A composite part must be comprised of documented basic parts.


Scars and Spacers

In your synthesized construct, you may have scars from your assembly method or intentional spacers between basic parts.

  • You should add novel scars to your composite parts by creating them as basic parts with a part type of "Scar."
  • You should add spacers to your composite parts by creating them as basic parts with a part type of "DNA."

You can then add these minimal basic parts into your composite part to ensure that it is sequence accurate.