Cell-free chassis/Vesicle

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Cell-Free Systems (back to Intro)	Introduction
Chassis description
Preparation protocol	Pictorial representation of method for vesicle-encapsulation. This method is based on Engineering Asymmetric Vesicles by Sophie Pautot et al (2003) and Toward an artificial cell based on gene expression in vesicles by Vincent Noireaux et al. (2005).
Preliminary Testing	Picture of a vesicle expressing GFP. Encapsulated within the vesicle is the Commercial E. coli S30 extract together with the DNA constuct BBa_T9002. The vesicle was seen under a phase-contrast microscope with white light (left picture) and with a filter (right picture).

References

Pautot S, Frisken BJ, and Weitz DA. Engineering asymmetric vesicles. Proc Natl Acad Sci U S A 2003 Sep 16; 100(19) 10718-21. doi:10.1073/pnas.1931005100 pmid:12963816. PubMed HubMed [1]
Noireaux V, Bar-Ziv R, Godefroy J, Salman H, and Libchaber A. Toward an artificial cell based on gene expression in vesicles. Phys Biol 2005 Sep 15; 2(3) P1-8. doi:10.1088/1478-3975/2/3/P01 pmid:16224117. PubMed HubMed [2]

All Medline abstracts: PubMed HubMed

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